Extraction, Separation and Antioxidant Activity of Polyphenols from Mango Pomace
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Using mango pomace as the raw material, the extraction method and process conditions of crude polyphenols were determined by single factor experiments. Crude polyphenols were extracted with organic solvents of different polarities to screen the components with the highest contents. These screened components were separated and purified by HPD100 macroporous resin. Furthermore, the purified components were qualitatively analyzed by LC-Q-TOF-MS, and comparison was made on the in vitro antioxidant activities of the extraction phases obtained by different solvents and the purified components. The results showed that ultrasonic-assisted extraction was more effective, with the optimal extraction conditions as: ethanol concentration 80%, extraction time was 1.5 h, extraction temperature was 60 ℃, ultrasonic power was 250 W, and the liquid-to-material ratio 30:1 (V/W). Under these conditions, the yield of polyphenols was 9.68%. The content of polyphenols extracted by ethyl acetate was the highest (254.41 mg/g). A total of 17 compounds were identified from the purified components obtained by resin, which mainly included flavones, flavonols, phenolic acids and tannins, and had excellent antioxidant activities. The IC50 values for the DPPH, ABTS+, hydroxyl and superoxide anion radical scavenging assays were 0.191×10-3, 0.081, 0.43 and 0.63 mg/mL, respectively. These results indicated that the purified components in the ethyl acetate phase were the material basis for the antioxidant activities of mango pomace.
