Inhibition of Proliferation and Apoptosis of Cervical Cancer Hela Cells by Treating with Moringa Oleifera Lam Alkaloids
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Abstract:
The aim of this study was to investigate the effects of Moringa Oleifera. Lam (M. oleifera) alkaloids on proliferation and apoptosis of cervical cancer Hela cells and its possible mechanism. Following 48h of treatment with M. oleifera alkaloids at various doses (20~320 μg/mL) , the proliferation, apoptosis and protein expression level of Hela cells were detected by MTT assay, colony formation assay, flow cytometric analysis and western blot. The results showed that the survival rate of Hela cells decreased gradually with the increase of M. oleifera alkaloids concentration. When the concentration of M. oleifera alkaloids was 160 μg/mL, the cell survival rate was 35.87% (p<0.001), the survival rate of human normal colon cell NCW460 is 91.91%. Colony formation experiments showed that M. oleifera alkaloids (40~160 μg/mL) significantly inhibited the colony formation of Hela cells, and the inhibition rates were 26.04% (p<0.05), 37.19% (p<0.01) and 67.77% (p<0.001), respectively. When the concentration of M. oleifera alkaloids was 80 μg/mL, the morphology of Hela cells changed significantly. Further flow cytometry analysis showed that the number of apoptotic cells in Hela cells increased with the increase of the concentration of M. oleifera alkaloid. When the cells were treated with 160 μg/mL of M. oleifera alkaloids for 48 h, the number of apoptotic cells was 42.1% (p<0.001). Further experiments showed that at 48 h, M. oleifera alkaloid up-regulated the ratio of Bax/Bcl-2 and the expressions of Caspase9 and down-regulated the expressions of P-Stat3 and CyclinD1 at the protein level in Hela cells. Our results indicate that M. oleifera alkaloids inhibits cell proliferation of Hela cells through the induction of apoptosis, which is mediated via inhibition of the Stat3-related pathway.
