Promoting Apoptosis of Lycium barbarum Polysaccharides of Hepatocarcinoma Cells in Rats
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Abstract:
To investigate the effect and mechanism of Lycium barbarum polysaccharides on apoptosis of model rats with hepatocarcinoma. Forty-five healthy male SD rats were selected, with 5 as the normal group, and the remaining 40 rats divided into liver cancer model group, drug control group, low- and high-dose intervention groups. The cell proliferation, apoptosis and cycle distribution of the rats in each group were observed, and the expressions of PTEN, p-Akt, mTOR, Bcl-2, Bax and caspase-3 were detected. The results showed that the proportion of cells at the G1 phase in the high-dose intervention rats was 57.59%, which was higher than those of the model group, drug control group and low-dose intervention group (p<0.05). The proliferation rate and apoptotic rate of the rats in high-dose intervention group were 8.53% and 47.68%, respectively, which were better than those of the model group, drug control group and low-dose intervention group. The relative expression of PTEN, p-Akt, mTOR, Bcl-2, Caspase-3 and Bax in the high-dose treated rats was 0.98, 1.05, 1.11, 1.26, 1.19 and 0.98, respectively. The interventional effect was greater than that of the drug control group and low-dose intervention group (p<0.05). The results showed that the Lycium barbarum polysaccharides could significantly regulate the proliferation, apoptosis and cell cycle distribution, as well as the expression of PTEN, p-Akt, mTOR, Bcl-2, caspase 3 and Bax in the rats with hepatocarcinoma. Lycium barbarum polysaccharides could block the cell cycle distribution, inhibit the proliferation and promote cell apoptosis of hepatocarcinoma cells, which provides a theoretical basis for the clinical treatment of hepatocarcinoma.
