Structural Analysis of the Degradation Products of Chondroitin Sulfate from Sturgeon Cartilage
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Abstract:
Using sturgeon cartilage as the raw material, chondroitin sulfate was extracted via the alkaline extraction-enzymatic-ethanol precipitation process, degraded by conventional comminution and superfine pulverization combined with irradiation technology, and then subjected to chemical and structural analyses of chondroitin sulfate using ion chromatography, ultraviolet-visible spectroscopy, infrared absorption spectroscopy and nuclear magnetic resonance spectroscopy. Monosaccharide analysis showed that the content of rhamnose in the crude sample increased from 56.16% to 59.31% after the co-treatment of superfine comminution combined with irradiation, and the content of glucuronic acid in the crude sample decreased from 33.69% to 31.84% after the co-treatment of superfine comminution combined with irradiation, The final purified samples contained 88.77% glucuronic acid. Ultraviolet spectroscopy revealed that the co-treatment by superfine pulverization combined with irradiation caused breakdown in the molecular structure of chondroitin sulfate i.e. the cleavage of glycoside bonds and the production of C=O groups. The results of infrared spectroscopy showed that the chondroitin sulfate treated by conventional comminution or the co-treatment of superfine comminution combined with irradiation treatment. The nuclear magnetic resonance analysis showed that the proton signals of acetylgalactosamine and glucose on chondroitin sulfate were enhanced after the treatment of superfine pulverization combined with irradiation. It is speculated that the chondroitin sulfate from sturgeon cartilage contained GlcA (β1→3) GalNAc-4SO3 and GlcA (β1→3) GalNAc-6SO3 repeating structural units.
