To investigate the effect of water extract of Rhizoma Arisaema on the expression of PKM2 and mTOR in gastric cancer rat cells. 50 SD male rats were selected, 10 of which were used as the normal group, and the remaining 40 were used to establish the gastric cancer model. The rats were divided into gastric cancer group, drug control group, low and high concentration group, and were treated with intervention. The proliferation and apoptosis of cancer cells in gastric cancer rats were observed, and the expressions of motilin, gastrin, PKM2, mTOR, bcl-2, PI3K and Akt in each group were detected. The results showed that the levels of motilin and gastrin in the high-concentration group were 137.65 pg/ml and 88.76 pg/ml, respectively, and the apoptosis rate was 49.73%, which were all higher than those in the gastric cancer group, drug control group and low-concentration group (p<0.05). The proliferation rate of rats in the high-concentration group was 7.55%, and the relative expression levels of PKM2, mTOR, bcl-2, PI3K and Akt were 1.37, 1.35, 1.37, 1.42 and 1.39, respectively, which were lower than those in the gastric cancer group, drug control group and low-concentration group (p<0.05). The results showed that the gastric function of gastric cancer rats was improved, the proliferation rate of cancer cells was decreased and the apoptosis rate was increased. The expression of PKM2, mTOR, Bcl-2, PI3K and Akt was obviously regulated by the water extract of rhizoma Arisaema. The anticancer ability of the water extract of Rhizoma Arisaema was concentration-dependent, indicating that the water extract of Rhizoma Arisaema could inhibit the proliferation of gastric cancer cells, promote apoptosis of gastric cancer cells and provide a theoretical basis for clinical treatment of gastric cancer.
[1] Li D, Cheng P, Wang J, et al. IRF6 is directly regulated by ZEB1 and ELF3, and predicts a favorable prognosis in gastric cancer [J].Front Oncol, 2019, 9:220
[2] Wang J, Wen T, Li Z, et al. MicroRNA-1224 Inhibits tumor metastasis in intestinal-type gastric cancer by directly targeting FAK [J]. Front Oncol, 2019, 4: 222
[3] Khosrow-Khavar F, Kurteva S, Douros A. Re: Metformin use and gastric cancer risk in diabetic patients after helicobacter pylori eradication [J]. J Natl Cancer Inst, 2019, 111(10): 1107-1109
[4] Uno Y, Kanda M. ASO Author reflections: increased expression of DNAJC12 is associated with aggressive phenotype of gastric cancer [J]. Ann Surg Oncol, 2019, 26(3): 592-593
[5] Chen D, Cao G, Liu Q. A-kinase-interacting protein 1 facilitates growth and metastasis of gastric cancer cells via slug-induced epithelial-mesenchymal transition [J]. Cell Mol Med, 2019, 23(6): 4434-4442
[6] Li Q, Ge Y, Chen X, et al. LEM domain containing 1 promotes proliferation via activating the PI3K/Akt signaling pathway in gastric cancer [J]. J Cell Biochem, 2019, 120(9): 15190-15201
Tang Jian-hua, Zhang He-ming, Dong Yun-cheng, et al. Experimental study on apoptosis of human gastric cancer BGC823 cells induced by Tiannanxing alcohol extract [J]. Shaanxi Traditional Chinese Medicine, 2011, 32(10): 1421-1422
Qiu Li-min, Jiang Shuang. Rhizoma Arisaema polysaccharide and cisplatin on proliferation, apoptosis and epithelial-mesenchymal transition of breast cancer MDA-MB-231 cells [J]. Traditional Chinese Medicine, 2016, 39(3): 630-633
Dong Wei, Zhang Bo, Shao Chao, et al. Inhibitory effect of water extract of Fresh Tiannanxing on uterine fibroma and estrogen in mice [J].Western Medicine, 2019, 31(5): 679-682, 688
Shi Yu-xiong, Guo Zhi-wei, Mu Li, et al. Expression and clinical significance of mir-185 and pKM2 in gastric cancer [J]. Journal of Central South Medical Science, 2016, 44(3): 267-270
[11] Gao S, Chen M, Wei W, et al. Crosstalk of mTOR/PKM2 and STAT3/c-Myc signaling pathways regulate the energy metabolism and acidic microenvironment of gastric cancer [J]. J Cell Biochem, 2018, 11
Xiao Lin, Zhou Yang-xiang, Tan Hua-liang, et al. Effects of ginkgo flavonoids on the expressions of VEGF, Bcl-2 and bax in rats with gastric cancer [J]. Modern Digestion and Interventional Diagnosis and Treatment, 2019, 24(3): 245-248, 253
Jing Huai-zhi, Qiu Feng, Chen Shi-zhi, et al. Expression of TRIM25 and PKM2 protein in non-small cell lung cancer [J]. Journal of Southern Medical University, 2015, 35(3): 437-441
Song Hui, Zhao Xu-dong, Ji Wen-yue, et al. The expression and clinical significance of PKM2 in laryngeal squamous cell carcinoma [J]. Chongqing Medical College, 2016, 45(15): 2080-2083
[15] Yu J S, Cui W. Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination [J]. Development. 2016, 143(17): 3050-60
Zhang Yong, Yang Chen, Wang Xin-zhi, et al. Effects of HDAC5 on proliferation and apoptosis of gastric cancer SGC-7901 cells [J]. Chinese Cancer Clinic, 2017, 44(10): 469-473
[17] Lv C L, Zhang T, Yan T Z, et al. Micro RNA-448 inhibits the regeneration of spinal cord injury through PI3K/AKT/Bcl-2 axis [J]. Eur Rev Med Pharmacol Sci, 2019, 23(7):2719- 2726
[18] Li Y, Lin Y, Huang X, et al. SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways [J]. Acta Biochim Biophys Sin (Shanghai), 2019, 51(6): 580-587
[19] Wang Z, Bao Z, Ding Y, et al. Nε-carboxymethyl-lysine- induced PI3K/Akt signaling inhibition promotes foam cell apoptosis and atherosclerosis progression [J]. Biomed Pharmacother, 2019, 115:108880
[20] Chen B, Shen Z, Wu D, et al. Glutathione peroxidase 1 promotes NSCLC resistance to cisplatin via ros-induced activation of PI3K/AKT pathway [J]. Biomed Res Int, 2019: 7640547
