In this study, the anti-inflammatory effect of tanshinone on knee osteoarthritis was studied based on the concentration of tanshinone and the changes of various indexes. Four groups of adjuvant arthritis rat models were constructed, each group was given 21 days. Knee osteoarthritis index in rats was determined by knee osteoarthritis evaluation criteria and knee bone diameter measurement. Isolated vascular ring perfusion and double-antibody sandwich abc-enzyme-linked immunosorbent assay were used to determine the content of acetylcholine-influenced endothelium-dependent relaxation function and von willebrand factor in each group. Cell fluorescence values were collected from the samples of tanshinone and the anti-inflammatory activity of tanshinone was determined by the activity of luciferase reporter gene. The results showed that the evaluation value of knee osteoarthritis in tanshinone group was about 5.26, which was lower than that in methotrexate group and fengyang ethanol extract group. After the injection of acetylcholine the relaxation rate was about 88.72 η/%, no significant fluctuations; The vascular haemophiliac factor content was approximately 35.22 λB/U?L-1, the closest to the control group. In the 9 samples of tanshinone, the luciferase reporter activity of 55% eluent was approximately 89.26, with the best anti-inflammatory effect, indicating that tanshinone can repair endothelial cells, and has a good anti-inflammatory effect on knee osteoarthritis model rats.
XI Jia-xi, ZHANG Hua-jun, CHEN Xiao-yu. Study on the protective effect of tanshinone ⅡA sulfonate injection on vancomycin-induced renal injury model rats and its mechanism [J]. China Pharmacy, 2016, 27(22): 3081-3084
WANG Dong, JI Xiao-wei, ZHANG Shuang. The protective effect of tanshinone ⅡA on rat models of uterine ischemia/reperfusion injury [J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2015, 19(27): 4384-4388
ZHANG Cai-hua, LI Cong, LI Hua-jun, et al. Effects of Tanshinone ⅡA on expression of transforming growth factor-β/Smads signaling pathway related factors in the liver tissue of rats with hepatic fibrosis [J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2015, 19(27): 4345-4350
SUN Lian-qing, CHEN Ying-ying, WANG Xuan, et al. Tanshinone ⅡA improves apoptosis and oxidative stress of Schwann cells induced by intermittenthigh glucose in suckling rats [J]. Chinese Journal of Diabetes Mellitus, 2015, 7(12): 748-753
ZHAO Mei, GUO Zhen-feng, LI Tian-shi, et al. Mechanisms of tanshinone ⅡA against arrhythmias in experiment myocardial infarction rat [J]. Chinese Journal of Hospital Pharmacy, 2016, 36(17): 1452-1455
GAO Xue-ke, WANG Dao, ZHANG Jing-yu, et al. Protective effect and its mechanism of tanshinone Ⅱ A on daunorubicin-induced cardiomyocyte injury in rats [J]. Chinese Journal of Cancer Prevention and Treatment, 2015, 22(23): 1797-1802
MA Yan-qiu, MENG Zhe, WANG Chen, et al. Effects of tanshinone ⅡA on cardiac fibrosis in rats and its mechanisms [J]. China Journal of Modern Medicine, 2016, 26(9): 23-28
LIU Ming-dong, SHEN Yong-hua. Effect of tanshinone ⅡA on cytokines of rats with severe acute pancreatitis lung injury [J]. Chinese Journal of Integrated Traditional and Western Medicine, 2015, 35(11): 1361-1366
LIN Si, QIN Fei, SONG Lu-yao, et al. Sodium tanshinone ⅡA sulfonate prevents postoperative peritoneal adhesions in rats by enhancing the activity of the peritoneal fibrinolytic system [J]. Journal of Southern Medical University, 2016, 36(2): 260-264
WANG Hua-min, MI Yi-qun, ZHANG Yong-liang, et al. Effect of warm acupuncture on behavior and serum IGF1 concentration in rats with knee osteoarthritis [J]. Journal of Southern Medical University, 2017, 30(5): 7-10
DONG Yi-xuan, LI Jing. Study on tanshinone ⅡA inhibiting the proliferation and migration induced by homocysteine of VSMCs and its mechanism [J]. China Pharmacy, 2016, 27(22): 3072-3076
MA Wen-jun, LIANG Ying. An experimental research on the analgesic effect and underlying mechanism of BTX-A for rat knee osteoarthritis [J]. Chinese Journal of Rehabilitation Medicine, 2017, 32(6): 649-653
ZHOU Zhi-jun. Establishment, verification and application of ELISA method for detection of von Willebrand factor antigen content [J]. Chinese Journal of Biological products, 2017, 30(10): 77-82
CHU Lei, ZHU Fen-yong, ZHOU Wen-jun, et al. Effect of baicalin on acute lung injury induced by intestinal ischemia/reperfusion in mice by inhibiting nuclear factor-κB activity [J]. Chinese Critically Ill First Aid Medicine, 2017, 29(3): 228-232
YE Da-lin, XIE Ping-jin, WEI He-wei, et al. Study on the mechanism of nourishing kidney and promoting blood flow decoction on the subchondral bone of rats with knee osteoarthritis [J]. Chinese Journal of Osteoporosis, 2018, 24(12): 1606-1611
