Anti-inflammatory Effect of Tanshinone on Knee Osteoarthritis Rats
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Abstract:
In this study, the anti-inflammatory effect of tanshinone on knee osteoarthritis was studied based on the concentration of tanshinone and the changes of various indexes. Four groups of adjuvant arthritis rat models were constructed, each group was given 21 days. Knee osteoarthritis index in rats was determined by knee osteoarthritis evaluation criteria and knee bone diameter measurement. Isolated vascular ring perfusion and double-antibody sandwich abc-enzyme-linked immunosorbent assay were used to determine the content of acetylcholine-influenced endothelium-dependent relaxation function and von willebrand factor in each group. Cell fluorescence values were collected from the samples of tanshinone and the anti-inflammatory activity of tanshinone was determined by the activity of luciferase reporter gene. The results showed that the evaluation value of knee osteoarthritis in tanshinone group was about 5.26, which was lower than that in methotrexate group and fengyang ethanol extract group. After the injection of acetylcholine the relaxation rate was about 88.72 η/%, no significant fluctuations; The vascular haemophiliac factor content was approximately 35.22 λB/U?L-1, the closest to the control group. In the 9 samples of tanshinone, the luciferase reporter activity of 55% eluent was approximately 89.26, with the best anti-inflammatory effect, indicating that tanshinone can repair endothelial cells, and has a good anti-inflammatory effect on knee osteoarthritis model rats.
