Comparative Study of Active Ingredients and Digestive Promotion Effect of Water- and Ethanol- Extract from Fufang Shanzha
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Abstract:
A method for the determination of chlorogenic acid and hesperidin in Fufang Shanzha was established in this work. And compare the content of active ingredients in Fufang Shanzha prepared by different solvents (ethanol and water) and their digestive promotion effect was investigated. The contents of chlorogenic acid and hesperidin were determined by HPLC. The comprehensive score was carried out based on their content and paste-forming rate. According to the principles offunctional food, the acute toxicity, the distance of ink movement in small intestine in mice, the body weight, food intakes, food efficiency ratio and the secretion amount of gastric juice and pepsin activity were evaluated. The results showed that the comprehensive score of active ingredients of water extraction (93.78) was higher than ethanol extraction (80.04). Water extraction and ethanol extraction had no acute toxicity to mice and rats. All of them could significantly increase the food intake of rats (p<0.05). The small intestine movement rates in middle dosage, high dosage group of water extraction and high dosage group of ethanol extraction increased by 26.88%, 28.00%, 26.55% (p<0.05, p<0.01, p<0.05), respectively. In addition, the secretion of pepsin in rats in middle dosage, high dosage group of water extraction and high dosage group of ethanol extraction increased by 38.59%, 53.29%, 36.64% (p<0.05, p<0.01, p<0.05), respectively. However, there was no significant difference in small intestinal movement rates and pepsin excretion of rats in other dose groups of ethanol extraction (p≥0.05). The activity of pepsin increased by 75.96%, 125.07%, 70.38%, 109.38% (p<0.05, p<0.01, p<0.05, p<0.01) in the middle dosage, high dosage group of water extraction and ethanol extraction, respectively. For the content of active ingredients and the digestive promotion effect, the water extract from Fufang Shanzha was superior to the ethanol extract.
