Study on Chemical Constituents of Ligustri Lucidi Fructus by UPLC-Q/TOF MS
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Abstract:
Using a LC-TOF/MS method, the chemical constituents of Ligustri Lucidi fructus were analyzed, and the transformation rule of the different constituents in the processing of Ligustri Lucidi fructus with the salt was discussed. A Sharpsil-H C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was composed of acetonitrile and water with 0.1% formic acid in gradient mode. The flow rate was 1 mL/min, the column temperature was 25 ℃ and the injection volume was 5 μL. An AB Sciex Triple TOF 5600 and ESI ion source was used for mass spectra. The MS data were collected in both positive and negative modes. The result showed that a total of 19 compounds were identified in the kidney-shape Ligustri Lucidi fructus, a total of 21 compounds were identified in the kidney-shape Ligustri Lucidi fructus after processing, a total of 28 compounds were identified in the round Ligustri Lucidi fructus, a total of 24 compounds were identified in the round Ligustri Lucidi fructus after processing. Through UPLC-Q/TOF MS technology, the analytical method of high sensitivity, high degree of separation and quick analysis was established for the chemical composition analysis of the raw and salt products of Ligustri Lucidi fructus. It was also proved that the components in various shapes of Ligustri Lucidi fructus were different in the processing with the salt, which will lay the foundation for the follow-up quality control and pharmacological research.
