Effects of Different Hydrolysis Methods on Antibacterial Activity and Tyrosinase Inhibition of Buckwheat Saponins
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Abstract:
A large amount of saponins in quinoa bran was found to have biologically active. The activity of tyrosinase inhibition and bacteriostatic of less polar saponins in quinoa bran were investigated by different hydrolysis methods, such as biphasic acid hydrolysis method, direct acid hydrolysis and enzyme hydrolysis bran. Their active component was been speculated and conducted qualitatively and quantitatively. Bacteriostatic activity was determined by minimum bacteriostatic concentration (MIC), minimum bactericidal concentration (MBC) and paper disk method. The whitening effect of tyrosinase inhibition was evaluated. LC-MS/MS and HPLC were used to identify the active compounds qualitatively and quantitatively. The results showed that the compounds that hydrolyzed and converted by bipolar acid had the best activities with MIC of 0.60 mg/mL, MBC of 1.20 mg/mL for Staphylococcus aureus and Salmonella enteritidis, MIC of 0.30 mg/mL, MBC of 1.20 mg/mL for Staphylococcus epidermidis. It also had certain inhibitory effect on Pseudomonas aeruginosa. The inhibition rate of 0.5 mg/mL of the conversion compounds to tyrosinase reached 68.09%. According to LC-MS /MS and HPLC analysis, some saponins were hydrolyze after two-phase acid decomposition, resulting in the decrease of the polarity of the compounds. Extraction of saponins from quinoa bran by biphasic acid hydrolysis could improve the activity of compound and enhance the function of the inhibitory on bacteria and rosinase activity. The extraction rate was significantly higher than that of direct acid hydrolysis and single enzyme hydrolysis.
