Antioxidant and Anti-aging Activity of the Flavonoids and Saponins from Rosa davurica Pall
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, the flavonoids and saponins from Rosa davurica Pall were found to have strong antioxidant capacity and exhibit dose-dependence to some extent in DPPH free radical scavenging assay, ABTS free radical scavenging assay, ferric-reducing antioxidant power (FRAP) assay, and total reducing power assays. The test samples at all the examined concentrations exhibited higher ABTS free radical scavenging rate than Vc, among which the saponin components in the 30% ethanol-eluted fraction (at 200 μg/mL) had an ABTS scavenging rate of 98.78%; At 800 μg/mL, the saponins in the 50% ethanol-eluted fraction had a DPPH free radical scavenging rate of 91.88% (which was very close to that for Vc). In FRAP assay, the reducing power of the flavonoids and saponins was higher than 1.50 which was very close to that for Vc (1.72). The total reducing power of the saponin components in the 30% ethanol-eluted fraction was 2.07 (which was close to that for Vc 2.28). In the aging model based on HaCaT cell repair, the flavonoids and saponins from Rosa davurica Pall were found having good anti-aging activity, and could repair the morphology of damaged cells, increased their survival rate, and showed dose-dependence to some extent. At 800 μg/mL, the repair rate of the four test samples (the 30% ethanol-eluted flavonoids, 50% ethanol-eluted flavonoids, 30% ethanol-eluted saponins, and 50% ethanol-eluted saponins) were 371.94%, 394.39%, 392.35%, and 399.83%, respectively, which were all close to that for Vc (404.25%).
