Study on Ability of Maca Alkaloids to Inhibit Tumor Formation in Colon Cancer by Regulatating Mouse Immunity
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Abstract:
The effect of maca alkaloids on the tumorigenic ability of mouse colon cancer CT-26 cells was investigated and its possible mechanism was explored. The animal model of colon cancer CT-26 cell transplantation in BALB/c mice was established and randomly divided into high dose maca alkaloid group, low dose maca alkaloid group and saline control group. The drug was continuously administered for 27 days. The weight gain curve of the mice and the volume growth curve of the transplanted tumor were drawn. The mice were sacrificed on the 28th day. The tumor inhibition rate, relative tumor proliferation rate and spleen index were calculated. The levels of serum IL-2 and IFN-γ were detected by ELISA. The histological changes of transplanted tumor, spleen, liver and kidney were observed by HE section. The results showed that, compared with the control group, the weight of the transplanted tumor in the experimental groups was significantly reduced (p<0.05).The tumor inhibition rates of the experimental groups were 43.37% and 45.26%, respectively. Histological observation showed that the necrotic area of transplanted tumor in the experimental group was significantly increased compared with the control group. The spleen index of the experimental group was significantly higher than that of the control group (p<0.05), and the white pulp proliferation of the spleen of the tumor-bearing mice in the experimental group was significantly larger than that in the control group. The serum IL-2 and IFN-γ levels in the experimental group were significantly higher than that in the control group (p<0.05). Results indicated that maca alkaloids could inhibit the tumor formation of mouse colon cancer CT-26 cells by increasing mouse immunity.
