Inhibitory Effect of Astragalus Polysaccharide on Uterine Leiomyoma Model Rats
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Abstract:
The inhibitory effect of Astragalus Polysaccharide on uterine leiomyoma model rats was investigated in this work. Fifty SD healthy female rats were selected, of which 10 were normal group, and the rest 40 were established uterine leiomyoma model and divided into model group and low, medium and high concentration group. The rats of each group were intervened to observe the apoptosis and cell cycle distribution of cancer tissue. The levels of estradiol (E2), progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH) and the expression of p53 gene, B lymphoma-2 (bcl-2), Bcl-2 related X protein (BAX) were detected. The results indicated that: the levels of E2, P, FSH and LH of high concentration group were 305.22 pg/mL, 44.21 ng/mL, 4.02 IU/L and 7.20 IU/L, respectively, which were significantly lower than those of model group, low and medium concentration group (p<0.05).The uterine weight and uterine coefficient of rats of high concentration group were 1.21 g and 5.44, respectively, which were significantly lower than those of model group, low and medium concentration group (p<0.05).The apoptotic rate of rats of high concentration group was 50.24%, and the percentage of cells in G1 phase was 62.41%, which was higher than the other three groups. The expression of p53 and Bcl-2 of high concentration group was 1.35 and 1.40, respectively, which was lower than that of model group, low and medium concentration group. The expression of Bax was 0.73, which was higher than that of model group, low and medium concentration group. The results showed that Astragalus polysaccharides could regulate the level of sex hormones, promote apoptosis of tumor cells, block cell cycle, and also regulate the expression of p53, Bcl-2 and Bax proteins in uterine leiomyoma model rats. Astragalus polysaccharides have a certain anti-tumor effect, and its ability showed a certain concentration-dependent.
