Protective Effect of Saponins from Panax japonicus on Brain Apoptosis in Mice with High-fat and High-fructose Diet
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Abstract:
The objective in this work was to investigate the effect of saponins from Panax japonicus (SPJ) on high-fat and high-fructose diet (HFFD) induced apoptosis in mice. KM mice were divided into normal control, high-fat and high-fructose-diet group, low-dose SPJ group (100 mg/kg) and high-dose SPJ group (300 mg/kg) and were fed with normal diet, high-fat and high-fructose diet (HFFD) and HFFD followed by SPJ at different doses (100 and 300 mg/kg) for one month, respectively. The protein levels of Bcl-2, Bax, Cytochrome C, Caspase-3, Caspase-12, COX-2, iNOS, IL-1β, NLRP3, Caspase-1, ASC, SIRT1 and p-AMPK were detected by western blot. The results showed that the protein levels of Bcl-2, SIRT1 and p-AMPK of high-dose SPJ group increased by 1.31, 1.05 and 1.33 times, respectively. The protein levels of Bax、Caspase 3、Caspase 12、Cytochrome C、COX-2、iNOS、IL-1β、NLRP3、Caspase 1 and ASC decreased by 34%、39%、60%、37%、41%、25%、43%、49%、31%、52%, respectively. In conclusion, SPJ have strong anti-apoptosis effect on mice with high-fat and high-fructose diet, and its mechanism is related with inhibition of NLRP3 inflammasome activitation, resulting in a reduction of expression of inflammatory factors, or the upregulation of the proteins associated with oxidative stress.
