Protective Effect of Gardenia jasminoides Extract on Dopaminergic Neurons in Mice with Parkinson's Disease Induced by MPTP
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Abstract:
In this study, mice with Parkinson's disease (PD) caused by MPTP were used as a model to systematically study the protective effects of Gardenia jasminoides extract from the medicinal and edible resources on the dopaminergic neurons in PD mice. The PD mice were administrated with Gardenia jasminoides extract (25, 50, 100 mg/kg) to detect the behavior. Detection of substantia nigra dopamine in mice by immunohistochemistry, to study the protective effect of Gardenia jasminoides extracts on dopaminergic neurons in PD mice. Compared with the model group, the climbing time of the low, medium and high dose groups decreased by 24.03% (p>0.05), 26.43% and 26.99% (both p<0.05) respectively; the rotational time increased by 64.16% (p<0.05), 77.46% (p<0.05) and 86.76% (p<0.05), respectively. The tensile strength increased by 16.95% (p>0.05), 22.56% (p<0.05) and 31.84% (p<0.05), respectively. The behavior of PD mice was significantly improved after Gardenia jasminoides extract treatments. The number of TH-positive cells in the Gardenia jasminoides extracts group was significantly higher than that in the model group. Compared with the model group, the DA of the low, medium and high dose groups increased by 100% (p<0.05), 138.46% (p<0.05) and 187.18% (p<0.05), respectively. The DOPAC increased by 52.63% (p<0.05), 89.47% (p<0.05) and 110.53% (p<0.05), respectively; In addition, compared with the model group, the serum levels of TNF-α, IL-1β, IL-6 in the low-dose group were reduced by 18.30% (p<0.05), 23.86% (p<0.05) and 26.03% (p<0.05), respectively. The data show that the Gardenia jasminoides extracts can significantly improve MPTP-induced behavioral changes in PD mice, protect dopaminergic neurons, and have good potential in the development of PD drugs.
