Analysis of Microbial Diversity in Badong Bean Paste by Illumina MiSeq High Throughput Sequencing Technology
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Abstract:
To better understand the microbial diversity of bean paste in Badong area of Enshi City, Hubei Province, Illumina MiSeq high-throughput sequencing technology was used to analyze the 16S rRNA gene sequence of bacteria and 18S rRNAV4 gene sequence of fungi in bean paste. The community structure and diversity of microorganisms in bean paste was compared. A total of 117644 valid sequences and 867 OTUS was obtained from the three samples. Diversity analysis showed that there was a high degree of microbial community diversity in bean paste. The analysis of microbial community composition indicated that Ascomycota (95.84%), Proteobacteria (47.13%), Firmicutes (32.37%) and Actinobacteria (12.87%) were dominant bacteria, accounting for more than 10.0%. There were 311 bacterial genera and 16 fungal genera identified in Badong bean paste samples. The average relative contents of dominant bacteria and fungi were 8.09% and 24.74%, respectively. The diversity of bacteria and fungi was significantly different at the generic level (p < 0.05). The results deepened the understanding of microbial community composition and diversity of bean paste, in order to provide a theoretical basis for the excavation and protection of microbial bacterial resources in bean paste in Badong area.
