A Fluorescence Detection Method for Melanin Content in Black-bone Silky Fowl Based on H2O2 Oxidation
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
For the first time, a simple and rapid fluorescence analysis method was established in this study for determining the melanin content in Black-bone Silky Fowl (BSF). The melanin in BSF could produce strong fluorescence after oxidation by H2O2 under alkaline conditions. In this study, the conditions for sample pretreatment and oxidation reaction were optimized, and the linear range, detection limit and spiked recovery rate were then evaluated. The results showed that the optimal oxidation conditions were: oxidation temperature 55 ℃, working concentration range of hydrogen peroxide solution 20%~25%, oxidation reaction time 2 h. The optimal sample pretreatment conditions were: NaOH concentration 0.1~0.2 M, ultrasonic time 40 min, ultrasonic temperature 70 ℃. The melanin concentration showed a good linear relationship in the range of 50~600 μg/mL, with the correlation coefficients as 0.9986. Compared with the traditional detection method, the fluorescence analysis method did not require the extraction of melanin from the sample, which greatly speeded up the analysis and simplified the analysis operation, and enabled at least 10 times lower detection limit as compared to the ultraviolet spectrophotometric method. The average recovery of the sample spiked with different concentrations of melanin working standard was 102.87% with the RSD value as 4.51 %. Using this method, the contents of melanin in BSF’s skin, thigh meat and breast meat were found to follow the order of chicken skin>thigh meat >breast meat (accounting for 1.08%, 0.44% and 0.34% of fresh weight, respectively). The above results showed that the established method is fast and accurate with a low detection limit, thus is suitable for the determination of melanin content in BSF.
