Screening of Culture Media and Optimization of Culture Conditions for Stropharia rugoso-annulata
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Abstract:
Stropharia rugoso-annulata has high nutritional value and medicinal value, but currently efforts on the screening of its culture medium and optimization of its culture conditions remain insufficient. In this study, the mycelial growth rate, growth potential and colony growth index of Stropharia rugoso-annulata were determined to screen the most suitable mother seed medium. The mycelial biomass was determined under different liquid medium formulas to screen the most suitable liquid medium for Stropharia rugoso-annulata. Based on the single-factor tests, the mycelial biomass of Stropharia rugoso-annulata was used as the response value, and the Box-Benhnken and central-composite method was applied to the optimization of liquid culture conditions for Stropharia rugoso-annulata. The results showed that the most suitable formula of the mother seed medium for the growth of Stropharia rugoso-annulata was: potato 200 g, dried rice straw 150 g, sucrose 20 g, corn flour 10 g, peptone 3 g, yeast extract 2 g, vitamin B1 5 mg, and water 1000 mL; The most suitable formula of the liquid medium for the growth of Stropharia rugoso-annulata was: sucrose 20 g, corn flour 30 g, straw powder 15 g, peptone 2 g, yeast extract 1 g, vitamin B1 5 mg, and water 1000 mL (natural pH); The optimal culture conditions for the liquid strain were: rotation speed 140 r/min, inoculum quantity 8%, incubation time 12 d. The mean of the actual mycelial biomass values obtained from three parallel experiments was 0.8108 g/100 mL, which was in agreement with the predicted value (0.8282 g/100 mL).
