Drug Resistance and Biofilm-forming Ability of Staphylococcus Aureus
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Abstract:
To study the drug resistance and biofilm-forming ability of Staphylococcus aureus (S. aureus), 70 strains of S. aureus were used as research objects. Their drug resistance spectra were analyzed by the paper diffusion method, while their biofilm-forming ability was examined using the Congo red plate test and crystal violet staining method. The biofilm formation related genes were detected by PCR, and the relationship between the drug resistance spectrum of these strains and their biofilm-forming abilities was analyzed. The results showed that the drug-resistance of S. aureus strains was more severe, of which 97.14% were resistant strains. All the strains could form biofilm, and the strains capable of forming biofilms with strong, medium and weak adhesive strength accounted for 71.43%, 18.57% and 10%, respectively. The detection rates of biofilm-related genes, fnbA, fnbB and clfB, were 57.14%, 20.00% and 44.29%, respectively. The strain with strong biofilm-forming ability had a broader spectrum drug resistance, and exhibited statistically significant (p<0.05) differences in the resistance rate to linezolid. The multi-drug resistance and ubiquitous biofilm of clinically-derived S. aureus have become a source of food safety hazards. The research results provide a theoretical basis for controlling S. aureus infections.
