Protective Effect and the Possible Mechanism of "Fresh Ginseng Paste" on Cisplatin-induced Kidney Injury in Mice
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Abstract:
The protective effect of fresh Ginseng Paste (FGP) on cisplatin (CDDP)-induced acute kidney injury in mice was studied here and its potential mechanism was explored. The renal function was evaluated by determining the serum levels of urea nitrogen (BUN) and creatinine (CRE) in mice. The oxidative stress level was evaluated by the detection of superoxide dismutase (SOD) and reduced glutathione (GSH) activity in kidney tissues and malondialdehyde (MDA) content. The pathological changes of renal tissues were observed by using H&E, TUNEL, Hoechst 33258 and immunohistochemical staining, and the apoptosis was analyzed by western blot analysis. The results clearly showed that compared with the normal group, the serum CRE and BUN in CDDP group increased by 96.97 μmol/L and 16.71 mmol/L, respectively. The MDA content in the renal tissues increased by 1.29 μmol/mg, and the GSH content decreased by 5.74 μmol/g, the activity of SOD was reduced by 49.94 U/mg (p<0.05). Compared with the CDDP group, FGP inhibited the overproduction of MDA, the increases of CRE and BUN. Moreover, FGP treatment increased the renal levels of GSH and SOD (p<0.05). FGP decreased the tissue glycogen deposition and the apoptosis of renal tubular epithelial cells (p<0.05). Western blot analysis indicated that FGP could significantly inhibit the overexpression of Bax and cleaved caspase-3 and reduce the protein expression level of Bcl-2. In summary, FGP exerted a protective effect on CDDP-induced renal injury in mice, and its mechanism may be related to the improvement of oxidative stress and anti-apoptosis.
