Comparative Analysis of Antioxidant Activity of Crude Polysaccharides from Eurotium cristatum Ascospores
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Abstract:
The ultrafine grinding method, ultrasonic method, and repeated freeze-thaw and grinding method were used to break the wall of Eurotium cristatum ascospore. The effects of these wall-breaking methods on the antioxidant activity of crude polysaccharides from the ascospore were investigated. The results showed that the wall-breaking rate decreased in the order of the repeated freeze-thaw and grinding method (93.73%), the ultrafine grinding method (82.27%), and the ultrasonic method (66.54%), with their corresponding extraction rates for crude polysaccharides as 2.18%, 2.06% and 1.84%, respectively, all of which were significantly higher than the extraction rate for the unbroken spores (1.15%). The results of the antioxidant tests showed that the crude polysaccharides obtained from the three types of broken ascospores exhibited a certain degree of anti-oxidative activity (which had a significant relationship with the mass concentration: dose-dependent effect). The crude polysaccharide obtained from the broken ascospores by ultrafine grinding showed the highest antioxidant activity, and at a mass concentration of 2.50 mg/mL, its capacity to scavenge DPPH, ABTS+ and ?OH radicals was 89.13%, 95.22% and 80.26% respectively with the reducing capacity for iron ions being 0.78. The antioxidant activities of the crude polysaccharides obtained by the repeated freeze-thaw and grinding method and the ultrasonic method were lower than that obtained by the ultrafine grinding method. These results indicated that the damage on the polysaccharide molecules of Eurotium cristatum ascospore due to the high-speed shear generated by this type of crusher at low temperature was less than that caused by the ultrasonic cavitation and repeated freeze-thaw process. Thus, the ultrafine grinding method is suitable for breaking the cell wall of Eurotium cristatum ascospores.
