Extraction, Isolation and Purification of Haw Polysaccharide and Its Antioxidant and Antiglycation Activities in Vitro
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, haw fruit was used as raw material to extract haw polysaccharide by hot water method. After decolorization and deproteinization, the polysaccharide was separated and purified using DEAE cellulose 52. On this basis, the monosaccharide composition and antioxidative and antiglycation activities of polysaccharide fractions were analyzed, and their structure-activity relationship was investigated. The results showed that the neutral polysaccharide WPS and the acidic polysaccharides SPS-1, SPS-2 and SPS-3 were obtained, and their yields were 46.33%, 7.86%, 37.94% and 7.12%, respectively. Monosaccharide composition analysis found that the main sugar composition of WPS was galactose, rhamnose, arabinose and glucose, while the sugar composition of SPS-2, SPS-1 and SPS-3 was mainly galacturonic acid, galactoserhamnose and arabinose, in which the content of galacturonic acid was 25.65%, 74.93% and 85.77%, respectively. Combined monosaccharide composition with infrared spectroscopy results, SPS-1, SPS-2 and SPS-3 could be pectin polysaccharides. The activity study showed that the antioxidant and antiglycation activities of salt-washed polysaccharides were stronger than that of water-washed polysaccharides, and the activity of SPS-3 was the strongest in salt-washed polysaccharides, followed by SPS-2 and SPS-1, which could be related to the higher galacturonic acid content and lower branching in the molecule. The results of this study are of great significance in the development of food-derived natural antioxidants and antiglycation agents.
