Physical and Chemical Properties of Polysaccharide Isolated from the Fruiting Bodies of Morchella importuna and Its Neuroprotective Effect
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Abstract:
The extraction, purification, structure and neuroprotective effects of a polysaccharide (MIP) purified from the fruiting bodies of Morchella importuna M. Kuo were investigated in this work. The polysaccharides of M. importuna were extracted with boiling water and then purified by DE-52 column and Sephadex G-100 column. The molecular weight and monosaccharide composition were analyzed by HPGPC and HPLC, respectviely. In order to investigate the neuroprotective role of MIP, the antioxidant activity of MIP against the H2O2-induced cytotoxicity and the underlying preventative mechanisms in rat pheochromocytoma (PC12) cells were illustrated. The results showed that the yield of MIP was 1.15%, the molecular weight of MIP was 1.35×106 U, the average particle size was 432.20 nm and the monosaccharide composition of MIP was mainly composed of xylose, glucose, galactose and a small amount of fucose. The heterocarbon of MIP was β configuration. MIP had excellent free radical scavenging activity and the IC50 value for the DPPH free radical was 1.18±0.04 mg/ mL. MIP could considerably enhance the viability of PC12 cells exposure to H2O2 and increase the activities of antioxidant enzyme like SOD, CAT and GSH-Px. It was worth noting that MIP could increase the activity of damaged SOD by 62.15%. It inhibited cell apoptosis via reducing caspase-3 activation and down-regulation Bax /Bcl-2 ratio. Accordingly, MIP could be used as a promising neuroprotective compound for nervous diseases treatment.
