Effect of Opuntia dillenii Polysaccharide on Apoptosis of Ovarian Cancer Tissue in Rats
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Abstract:
To investigate the effect of Opuntia polysaccharide on apoptosis of ovarian cancer rats, fifty SD healthy female rats were selected, of which 10 were normal group, the remaining 40 were used to establish ovarian cancer model and divided into model group and low, medium and high concentration cactus group. The rats in each group were intervened to observe the apoptosis and cell cycle distribution of cancer tissue. The expression of Bcl-2, PI3K, Akt, PTEN, p-Akt and mTOR protein were also detected. The results showed that the volume and weight of tumors in high concentration cactus group were 179.63±51.82 V/mm3 and 0.30±0.05 W/g, respectively, which were lower than those in other groups. The apoptotic rate and G1 phase cell proportion in high concentration cactus group were 19.22% and 62.33%, respectively, which were higher than those in other groups. The relative expression scores of Bcl-2, PI3K, Akt, p-Akt and mTOR in high concentration cactus group were higher than those in other groups, which were 1.37, 1.45, 1.40, 1.35, 1.39, respectively. The relative expression of PTEN was 0.76, which was higher than that of model group and low and medium concentration cactus group (p<0.05). Under the intervention of Opuntia polysaccharide, the apoptotic rate of ovarian cancer model rats increased and cell cycle was blocked. The expression of apoptotic related proteins Bcl-2, PI3K, Akt and cyclin PTEN, p-Akt and mTOR were regulated, and their ability was concentration-dependent. It could provide some theoretical guideline for the clinical treatment of ovarian cancer.
