Inhibition of Different Polysaccharides on H2O2-induced Skin Cell Aging
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Abstract:
The purpose of this study was to determine whether the effect of mixed polysaccharides in inhibiting skin cell senescence is superior to that of single polysaccharides by using different concentrations of H2O2 to induce damage to human skin fibroblasts (HSF) and human keratinocytes (HaCaT), on this basis, MTT assay and biochemical kit were used to detect cell viability and superoxide dismutase (SOD), malondialdehyde (MDA) and hydroxyproline (HYP). The survival rates of HaCaT and HSF cell peroxidation models established in this experiment were about 72.00% and 65.00%, respectively. The study found that the survival rate of the two single aging models was not statistically different from that of the model group (p>0.05). The contents of SOD, MDA and HYP were not significantly different from those of the model group except for individual indicators (p>0.05). In the HaCaT cell aging model, the survival rate of the mixed polysaccharide group was between 74.93% and 80.43%, the content of SOD was between 6.99 U/mg and 7.56 U/mg, the content of MDA was between 7.44 μmol/g and 8.41μmol/g, and the content of HYP was between 2.30 μmol/g and 2.43 μmol/g. In the HSF cell aging model, the survival rate of the mixed polysaccharide group was between 76.23% and 78.14%, the content of SOD was between 6.30 U/mg and 8.90 U/mg, the content of MDA was between 7.03 μmol/g and 8.93 μmol/g, and the content of HYP was between 2.19 μmol/g and 2.53 μmol/g. The above indicators were statistically significant compared with the model group (p<0.01 or p<0.05).Therefore, the overall improvement effect of the mixed polysaccharide group was more significant, which can improve the survival rate of senescent epidermal cells, increase the SOD, HYP content and reduce the MDA content of model cells. The study suggested that in the anti-H2O2-induced HSF and HaCaT cell oxidative senescence experiments, the mixed polysaccharides of different polysaccharides were significantly more effective than the single polysaccharides alone, at the same intervention concentration.
