Evaluation on the Antioxidant Activity of VPD and KGP in Jiupei by HepG2 Cells Model
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Abstract:
In this study, the cells-level antioxidant activities of two peptides, Val-Pro-Asp (VPD) and Lys-Gly-Pro (KGP), which were extracted, isolated and identified from Jiupei of Baijiu, were studied. The antioxidative activities of two peptides were evaluated by measuring their effects on intracellular reactive oxygen species (ROS) content, activity of antioxidant enzymes and other indexes in AAPH induced oxidative-damaged human hepatoma cells (HepG2 cells) model. The result showed that VPD and KGP possessed intracellular antioxidant activity. The mechanism of the action was that they can significantly decrease the content of ROS in cells, increase the activity of three enzymes of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reduced the glutathione (GSH) content. At the same time, they can also decrease the level of lipid peroxide in cells e.g. MDA content to protect cells from oxidative damage and capacity of two peptides basically increased with the rise of concentration. This provides the possibility to improve the contents of functional peptides from Jiupei into Baijiu by optimizing the distillation condition.
