A Liquid-phase Microextraction Method Based on Deep Eutectic Solvents Determination for Five Kinds of Fungicides Residues
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Abstract:
A method was developed for the determination of five fungicide residues (triadiconazole, pyrimethanil, triadimefon, carbazole, and cyprodinil) by high performance liquid chromatography with deep eutectic solvent as extraction in liquid-phase microextraction. A DES containing phenol as a hydrogen bond donor and choline chloride as a hydrogen bond acceptor was used as the extraction solvent. THF was used as a dispersant solvent to form small droplets of turbidity. The target analyte was extracted into trace DESs phase. After centrifugation, the upper DESs phase was subjected to high-performance liquid chromatography for analysis. Various parameters affecting extraction efficiency were investigated and optimized. The results were shown that the DESa molar ratio of choline chloride and phenol 1:2 is the best extraction efficiency. Under the optimum conditions are as follows: sample volume 5 mL, DES2 150 μL, and THF 150 μL, ultrasonic time 20 min and centrifugation time 10 min. The recoveries were between 73.7%~102.3%, the relative standard deviations (RSDs) were between 0.7%~7.1%, and the low limits of detection (LOD) were 0.026~ 0.052 μg/L.
