Optimization of the Extraction of the Volatile Substances in Cyclocarya paliurus Tea Based on GC-TOF-MS
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Abstract:
In order to optimize the key variables for the extraction of the volatile substances in Cyclocarya paliurus tea and further provide theoretical methods for the identification of the volatile substances, headspace-solid phase microextraction (HS-SPME) was used in this study to extract volatile substances from Cyclocarya paliurus tea before analysis by high-throughput gas chromatography-time of flight-mass spectrometry. The optimum conditions for HS-SPME were determined by designing different SPME fiber assemblies, extraction temperatures, extraction times and tea-to-water ratios. The results revealed the optimal extraction conditions: the use of CAR/PDMS (75 μm) as SPME fiber for a 60-min extraction at 80 ℃ and a tea-to-water ratio of 1:15. Under these conditions, 229 volatile substances were detected，including acids, aldehydes, ketones, alcohols, esters, alkanes, olefins and heterocyclic compounds. Among them, aldehydes, heterocyclics and alcohols had relatively high contents, with the top five contents derived from hexanal, 1-carophyllene, n-hexanol, 2-methylpropenal, and 2-hexenal.
