Inhibitory Effect of Phlorizin and Young Apple Polyphenols on Tyrosinase Activity
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Abstract:
To compare the potential of young apple polyphenols and phlorizin as tyrosinase inhibitors, the inhibitory effect and kinetics of phlorizin, young apple polyphenols and kojic acid on tyrosinase activity were analyzed and compared by spectrophotometry, in which kojic acid was used as a positive control. Results: phlorizin, young apple polyphenols and kojic acid could effectively inhibit tyrosinase activity. The order of their inhibitory effects was kojic acid>young apple polyphenols>phloridin, and their IC50 for monophenolase activity was 55.80, 54.30 and 23.81 μg/mL, respectively, and their IC50 for diphenolase activity was 75.30, 56.15 and 23.45 μg/mL, respectively. The phlorizin showed mixed inhibition at a concentration lower than 200 μg/mL and competitive inhibition at a concentration of 200 μg/mL, the young apple polyphenols showed non-competitive inhibition and the kojic acid showed mixed inhibition. In summary, the inhibitory effect and inhibitory mechanism of the inhibitor is not only related to its composition, but also related to its concentration. All three inhibitors can reduce the synthesis of melanin by inhibiting the hydroxylation of tyrosine to dopa and the oxidation of dopa to dopaquinone and dopachrome. Compared with phlorizin, young apple polyphenols is more suitable as tyrosinase inhibitors and has great potential for application in whitening products because of its better inhibitory effect, cheaper price and richer content.
