A New Method for the Determination of Clenbuterol Based on Aptamer and Gold Nanoparticles
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Abstract:
To detect clenbuterol (CL), a β-Agonist,a visual and rapid method based on aptamer and gold nanoparticles (AuNPs) was established in this study. The aptamer (DNA1) conjugating with clenbuterol, and its complementary DNA (DNA2) were designed and synthetized, respectively. The aptamer-AuNPs probe (probe1) and aptamer complementary DNA-AuNPs probe (probe2) were also synthetized in present study. In the presence of CL, aptamer bound to the target, AuNPs maintained a free state, however, these AuNPs began to aggregate and change color from red to purple in a high concentration of salt. In the absence of CL, aptamer and its complementary DNA formed a stable network, keeping red color. Some parameters, such as the concentration of aptamer, aptamer complementary DNA and salt were optimized, respectively. The results showed that in the optimum conditions, clenbuterol had a good linear relationship ranging from 1 to 1000 ng/mL, with correlation coefficients (R2) 0.991. The visual detection limit was 1 ng/g, and the recoveries at three spike levels ranged from 83.5% to 101.8%.This newly developed method was simple, sensitive, and reliable. It was suitable to detect clenbuterol in actual samples.
