Detection and Quantification of Sweet Potato Components by Droplet Digital PCR
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Abstract:
In this work, primers and probes were designed based on the specific fragment of Ipomoea batatas beta-amylase gene (β-AMY), and a droplet digital PCR method for detection of sweet potato was established. The specificity, sensitivity and adaptability of this method were tested. The results showed that the established method was specific to the detection of sweet potato components. The limit of quantitation (LOQ) and limit of detection (LOD) were 2.53 copies/μL and 0.67 copies/μL, respectively. According to the established linear relationship between copy number concentration (copy number/μL) and the sweet potato quality (mg), the quantitative analysis of 5% and 25% of the sweet potato mass percentage was performed, and the sweet potato content converted according to the linear relationship was 4.15% and 21.33%, respectively. The recovery rates were 83.06% and 85.31%, respectively, and the RSD values were between 6.33% and 6.95%. It showed that this method can accurately quantify sweet potato components with a mass percentage of 5% or more and it indicated that ddPCR method had strong potential in quantifying sweet potato. This method can provide technical support for market supervision of sweet potato and its products.
