Determination of 8 Phenols in Bakery Food and Raw Material by Isotope Dilution - Dispersive Solid Phase Extraction Couple with UHPLC-MS/MS
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Abstract:
A method based on isotope dilution (ID), dispersive solid phase extraction (DSPE) couple with UHPLC-MS/MS was presented for determination of 8 phenols, including 4,4’-Isopropylidenediphenol (BPA), 4, 4'-(1-methylpropylidene) bisphenol (BPB), 4,4'- (2,2-Dichlorovinylidene) bisphenol (BPC), 4, 4'-methylene diphenol (BPF), 4, 4'-Sulfonyldiphenol (BPS), 4,4'- (Hexafluoroisopropylidene) diphenol (BPAF), 2,4-Dihydroxybenzophenone (UV-0) and 2,4-Dihydroxybenzophenone (TH1790) in bakery food and raw material (Flour, Flour-improving Agent, Egg Yolk filling et al.). Target compound was extracted by acetonitrile, and purified by C18 dispersion adsorbent (for high oil content such as egg yolk filling, extra fats were removed beforehand by hexane). A total of 8 targets were separated on a C18 column by gradient elution with acetonitrile-ammonium hydroxide as mobile phase, ionized with negative electrospray ionization (ESI -), detected under the schedule of multiple reaction monitoring (schedule-MRM) mode, and quantified with internal standard method. A favorable linearity was acquired in the concentration range of 1.0~200.0 μg/L with the correlation coefficients (R2) better than 0.999, and the limits of detection (LODs) were ranged from 0.5 to 5.0 μg/L. The spiked average recoveries presented 82.10%~113.20%, with the relative standard deviation (RSD, n = 6) of 2.70%~9.50%. These methods are rapid, sensitive and suitable for the determination of 8 Phenols in bakery food and raw material.
