Analysis of the Chemical Constituents of Bupleurum Saponins in Bupleuri radix with Turtle Blood by UPLC/Q-TOF-MS/MS
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Abstract:
A rapid method for the analysis of saponins based on UPLC/Q-TOF-MS/MS was established by using Bupleuri radix with Turtle Blood processed products as raw materials. The analysis was performed on an Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) by gradient elution. The mobile phase consisted of 0.01% formic acid solution (A)-acetonitrile (B) at a flow rate of 0.3 mL/min, and the injection volume was 2 μL, the column temperature was at 40 ℃.The mass spectrometer was operated in negative mode. Results showed that the compounds with a mass error of less than 5×10-6, a correct isotope distribution and fragment ion were used as the target compounds, using the established screening database. Combined with software Formula Finder, Mass Calculators and other functions, online databases (Human Metabolome Database, PubChem, MassBank, ChemSpider, etc.), and two stage fragmentation rule, a total of 44 saponins were separated and identified in Bupleuri radix with Turtle Blood, including epoxy ether type, isophthalene type,12-ene-type and 12-ene-28-carboxylic acid type saikosaponins. In conclusion, the results demonstrate that UPLC/Q-TOF-MS/MS method is quick, accurate, and efficient for the identification of saponins in Bupleuri radix with Turtle Blood, and provides a feasible method for the selection of quality control indicators and the efficacy of active compounds in Bupleuri radix with Turtle Blood.
