Study on Degradation of Aflatoxin B1 during the Preparation of Rice Bran Polypeptide by Response Surface Methodology Microwave-assisted Enzyme Treatment
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To provide new pathways for detoxification of rice bran, the effect of microwave combined with enzymatic hydrolysis on aflatoxin B1 (AFB1) were investigated in rice bran. On the basis of single factor experiment, the factors of material-liquid ratio (X1), microwave time (X2), alkaline protease hydrolysis time (X3) and enzymatic hydrolysis pH (X4) were combined. Design Expert software and Box-Behnken central combination test method were used to optimize the degradation conditions of AFB1 and determine the removal amount of rice bran polypeptide AFB1. The initial concentration of AFB1 in rice bran was 100.14 μg/kg, and the optimum technological conditions were determined by response surface analysis: microwave power 750 W, microwave time 9.56 min; alkaline protease hydrolysis temperature 50 ℃, enzymatic hydrolysis pH 10.74, enzymatic hydrolysis time 1.57 h, enzymatic dosage 0.4%; material-liquid ratio 1:9.96. Under these conditions, the degradation rate of AFB1 in rice bran was 98.68%, and the residual concentration of rice bran polypeptide (1.85 μg/kg) was obtained, which was in agree with the national standard (<10 μg/kg). Compared with single factor treatment, microwave combined with enzymatic method can play a synergistic role and effectively reduce the content of rice bran AFB1. For the process was under mind condition, simple and efficient, it could be used for the aflatoxin contaminated crops.
