GC-MS Analysis of Volatile Oil from Artemisia argyi and Its Antioxidant Activity in Vivo
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Abstract:
In this paper, the GC-MS analysis of volatile oil from Artemisia argyi and its antioxidant activity in mice were studied. The volatile oil in Artemisia argyi was extracted by steam distillation, and the volatile oil of Artemisia argyi was analyzed by GC-MS. The results showed that eight components were identified from Artemisiaargyi, mainly eucalyptus brain, ketone, alcohol, and alkane. The aging model was established by intraperitoneal injection of D-galactose for six weeks, and the contents of SOD, GSH, PCO and MDA in the heart, liver and kidney of mice were measured and the morphology of mouse organs was observed. The results showed that the SOD and GSH contents in the heart, liver and kidney of the aging model group were significantly lower than those in the blank control group (p<0.05, p<0.01, p<0.001), and the MDA and PCO contents were significantly increased (p<0.001), the four indicators were much more affected in cardiac organs, SOD and GSH levels were reduced by 57.72% and 44.58%, respectively, and MDA and PCO levels were increased by 43.59% and 86.49%, respectively. The aging mouse model was successfully constructed. Compared with the aging model group, the SOD and GSH contents in the heart, liver and kidney of the wild wormwood volatile oil protection group were significantly increased (p<0.05, p<0.01, p< 0.001), MDA and PCO levels were significantly reduced (p<0.05, p<0.01, p<0.001), of which SOD content had a greater effect on the kidney, increased by 80.42%; GSH and MDA content had a greater impact on the heart, increased by 73.26% and decreased by 46.93%, respectively. The PCO content had a greater impact on the liver, which was reduced by 47.16%. It indicated that the volatile oil of Artemisia argyi has certain anti-oxidation effect in mice. The results of pathological section showed that the volatile oil of Artemisia argyi could protect the morphological integrity of oxidatively injured mouse cardiomyocytes, hepatocytes and kidney cells.
