Abstract:To study the mechanism of Gastrodia elata extract on anti-hypoxia ability in rats. The rats were randomly divided into normal group, positive control group, low-dose Gastrodia elata extract group and high-dose Gastrodia elata extract group. The body weight, survival time and survival rate of rats were investigated. Malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lactate dehydrogenase (LDH), lactate (LA), glutathione (GSH), glutathione reductase (GR), heat shock protein 70 (HSp70), endothelin-1 (ET-1), nitric oxide synthase (iNOS), the expression of related proteins for hypoxia inducible factor-1alpha (HIF-1a) were detected. The results showed that, compared with the normal group, the survival time of rats in the high dose Gastrodia elata extract group increased by 34.01%, the levels of LDH (8614.24 nmol/mL), LA (2.01 nmol/mL), GSH (965.5 mg/mL), GR (0.30 U/mL) increased (p<0.05), HSp70 (4.14), MDA (63.52 U/mL), CAT (23.24 U/mL), ET-1 (1.96), iNOS (2.10), HIF-1a (0.28) decreased (p<0.05). These results suggested that Gastrodia elata extract can effectively improve the antioxidant capacity of rats, the level of oxidative stress in rats, and thus improve the hypoxic capacity of rats.
