Brain Protective effect of Lilium lancifolium Hypericin on Rats with Cerebral Ischemia Reperfusion Injury
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Abstract:
The brain protective effect of Lilium lancifolium hypericin on the hippocampal tissue of the rats with cerebral ischemia reperfusion injury was investigated. The 50 male SD rats were randomly divided into sham group (S), model group (M), hypericin low (25 mg/kg), middle (50 mg/kg) and high (100 mg/kg) dose groups in this work. Except S group, other groups were given the modified Longa line blockage method to establish the left middle cerebral artery occlusion (MCAO) model. After 24 hours of modeling, the learning and memory ability of rats were tested by Morris water test, and brain tissue was taken for pathological HE staining to evaluate the damage of neurons in the hippocampus. Western-blot was employed to detect the expression of BDNF and p75NTR in hippocampus. The results showed that high-dose hypericin could significantly shorten the escape latency times (15.45±1.86) and increase the number of crossing the platform (8.67±1.52) times for the ischemia-reperfusion rats, which was statistically different from that in the M group (p<0.05). In addition, the pathological damage in the hippocampal area was improved, so that the nerve cells were arranged orderly, with clear structure and basically intact and normal morphology. It can also regulate the increase of BDNF expression and the decrease of p75NRT lever in hippocampal tissue (p<0.05). Hypericin can significantly improve the cognitive impairment caused by ischemia reperfusion injury and improve the learning and memory ability. Moreover, it has a certain brain protective effect, which might be caused by the up-regulation of BDNF and down-regulation of p75NRT expressions to reduce the damage of neurons and neurons in the hippocampus.
