Bioaccessibility of Different Speciation of Chromium in Auricularia auricula during the in Vitro System
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Abstract:
The in vitro system was used to simulate the digestion of Auricularia auricula in stomach and intestine to analyze the bioaccessibility of chromium. The contents of chromium with different speciations were determined by HPLC-ICP-MS. The 29 samples were simulated the digestion by in vitro system. The bioaccessibilities of chromium in the stomach were ranged from 15.10% to 35.87%, and from 11.02% to 20.36% in the intestine. The contents of chromium in stomach were more than that in the intestine. The speciation of chromium mainly was trivalent, which proved that Auricularia auricula could absorb Cr(Ⅵ). The standard solutions of 0.5 μg/L Cr(Ⅲ) and Cr(Ⅵ) were mixed to determined for seven times, which RSD was 2.55%~4.98%. Six parallel experiments were repeated to verify the precision of the method, which relative deviation of the results was less than 9.5%. It was put forward that the bioaccessibility of chromium in Auricularia auricula was Cr (Ⅲ), which was benefit for the body. The Auricularia auricula could absorb Cr(Ⅵ) which do harm to the health. This experiment explored the change of the chromium speciation in Auricularia auricula during the process of digestion in stomach and intestine, which provided a reference for the study on the limit standard of the chromium in Auricularia auricula.
