Comparison of the Volatile Components in Leaves, Flowers and Stems of Loquat by HS-SPME-GC-MS and Principal Component Analysis
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Abstract:
The volatile components in the leaves, flowers and stems of loquat were analyzed and compared using headspace solid-phase microextraction and gas chromatography-mass spectrometry. The relative contents of individual components were calculated by the area normalization method while the principal components were also analyzed. Twenty nine compounds were identified in loquat leaves, accounting for 91.16% of the total volatile components; Twenty seven compounds were identified in loquat flowers, accounting for 99.99% of the total volatile components; Nineteen compounds were identified in the stems of loquat, accounting for 90.05% of the total volatile components. A total of 55 compounds were identified from the leaves, flowers and stems of loquat, among which 6 compounds were in common. The loquat leaves, flowers and stems had 23, 21 and 13, respectively, their own characteristic compounds. Two principal components were found to represent the 55 volatile components in the 3 types of samples. The Principal Component Analysis revealed a great difference in the comprehensive score among the samples, with the comprehensive score decreasing in this order: leaves > flowers > stems. The experimental results showed large differences in the species and contents of the volatile components among the leaves, flowers and stems of loquat.