Physicochemical Properties and in Vitro Bioactivity of Sulfated Pleurotus eryngii Polysaccharide
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Abstract:
In this work, Pleurotuseryngii polysaccharide (PEP) was chemically modified by chlorosulfonic acid-pyridine method, and three kinds of sulfated Pleurotuseryngiipolysaccharides (SPEP) were obtained. According to the volume ratio of chlorosulfonic acid to pyridine (1:2, 1:5 and 1:8), the three kinds of SPEP were recorded as SPEP-601:2, SPEP-601:5 and SPEP-601:8, respectively. The physicochemical properties of PEP and SPEP were detected by chemical methods, gas chromatography and gel permeation chromatography. In addition, the antioxidant activities (DPPH· scavenging ability and O2-· scavenging power) and the inhibitory effect on key enzymes (α-amylase and α-glucosidase) for carbohydrate digestion and absorption of PEP and SPEP were also determined. The results showed that the degree of substitution of SPEP-601:2, SPEP-601:5 and SPEP-601:8 were 0.26±0.00, 0.21±0.00 and 0.19±0.00, respectively. All the SPEP had less total sugar, uronic acid and lower molecular weight than the PEP. They had the same monosaccharide composition with different molar ratio. Moreover, SPEP-601:8 possessed the best scavenging capacity for DPPH compared with other SPEP. SPEP-601:2 showed the strongest scavenging effect on O2- ·. All the SPEP had higher α-amylase and α-glucosidase inhibitory activity than the PEP, increasing at least by 834.33% and 97.66%, respectively. In addition to DPPH radical scavenging capacity, SPEP exhibited stronger biological activity than PEP at the same dose. These results suggested that sulfation modification might be an effective way to enhance antioxidant and hypoglycemic activities of the polysaccharide.
