Abstract:In this study, chitosan/polyvinyl alcohol nanofibers (CS/PVA) with different mass ratios were prepared using electrospinning. Morphology differences of nanofibers were analyzed by scanning electron microscopy. The CS/PVA nanofibers with good morphology was selected (mass ratio was 1/9) to prepare lysozyme/chitosan/polyvinyl alcohol nanofibers (LZ/CS/PVA) by adding different concentrations of lysozyme. Morphology and characteristics of LZ/CS/PVA nanofibers were analyzed by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and differential scanning calorimetry. The results showed that the addition of lysozyme affected the morphology uniformity of fibers and the degree of adhesion between fibers. With the addition of lysozyme, the average diameter of fibers decreased from 339.62±92.72 nm (CS/PVA) to 262.10±86.32~283.01±96.83 nm (LZ/CS/PVA series fiber). When the concentrations of lysozyme were 0.3% and 0.5%, the LZ/CS/PVA nanofibers possessed good appearance. There existed molecular interaction among the components of the nanofibers hindering the formation of crystals, resulting in an almost amorphous structure. The addition of LZ increased the thermal stability of the nanofibers, and the melting point rose from 185.60 ℃ to 189.30~191.00 ℃. LZ/CS/PVA nanofibers are prepared from safe and non-toxic materials, which have potential applications in the food industry.
