Bioactive Compounds Extracted by Different Solvents from Rosa acicularis ‘Luhe’ Leaves and Their Antioxidant Activity
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Abstract:
In this study, the extraction of bioactive compounds from Rosa acicularis ‘Luhe’ leaves using ultrasonic-assisted method with different solvents (distilled water, absolute ethyl alcohol, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethyl alcohol, 80% ethyl alcohol, 1-butyl alcohol, acetone and ethyl acetate) was studied. Total flavonoids, total phenols, procyanidins, and antioxidant activity (DPPH radical scavenging capacity, hydroxyl radical scavenging capacity and total reducing power) were first evaluated in the extracts of Rosa acicularis ‘Luhe’ leaves. Correlation analysis of phytochemical contents with antioxidant activity was evaluated. Results indicated that different solvent extraction effects were different. The extract with ethanol concentration of 60% V/V possessed the highest yields (23.76±0.04)% and the highest total flavonoids and total polyphenol content (74.29±0.01) mg/g and (24.02±0.24) mg/g, respectively. The highest procyanidins content (12.92±0.46) mg/g was obtained in ethanol concentration of 80% V/V. The bioactive compounds contents were positively correlated to the antioxidant activity. Total phenols and total flavonoids are the major contributors to the antioxidant activities of Rosa acicularis ‘Luhe’ leave extracts.