Chemical Composition and Antioxidant Activity of Cornus officinalis
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Abstract:
To clarify the chemical composition and antioxidant activity of Cornus officinalis, a dual-use species for medicine and food, the ethanol extract of Cornus officinalis was used as raw material in this research for examining the antioxidant components in the 20% ethanol elution of the ethanol extract through macroporous adsorption resin. The antioxidative fraction of the alcohol extract was subjected to systematic isolation and purification by chromatography techniques such as macroporous resin column chromatography, normal phase silica gel column chromatography, Sephadex LH-20 column chromatography, flash reversed phase silica gel column chromatography, and preparative HPLC chromatography. Based on the data obtained by nuclear magnetic resonance spectroscopy and mass spectrometry and from literature, five compounds were isolated and identified from the ethanol extract of Cornus officinalis: 7-β-O-ethylmorroniside (1), 7-α-O-ethylmorroniside (2), 3-O-caffeoylquinic acid n-butyl ester (3), (7R, 9R, 10R)-3, 9-di-hidroxicalameneno (4), and vomifoliol (5), among which compounds 3-5 were isolated for the first time from Cornus officinalis. In the meantime, the antioxidant activity of the isolated monomeric compound was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and Ferric ion reducing antioxidant power (FRAP) assays. Compounds 1-3 all showed certain antioxidant activities in the DPPH and FRAP experiments, with Compound 3 exhibiting the highest antioxidant activity.
