Effects of Mulberry Leaves Water Extract on Cholesterol Metabolites in Feces of High-fat Diet Fed Mice
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Abstract:
In order to provide data support for hypolipidemic function of mulberry leaves, the effect of water extract of mulberry leaves on cholesterol metabolites in feces of high-fat diet mice was studied. The water extract of mulberry leaves was set to three dose groups of high (8.0 mg/kg·bw), medium (4.0 mg/kg·bw) and low (2.0 mg/kg·bw) according to their DNJ content. The mice were fed with high-fat diet for 4, 8, 12, and 16 weeks. The serum pH, fecal sterol, bile acid, and short-chain fatty acids (SCFAs) were measured in each group, and correlation analysis between SCFAs and serum cholesterol (Total Cholesterol, TC) levels and atherosclerosis index (AI) were analyzed. Compared with the high-fat control group, the water extract of mulberry leaves (8 mg/kg·bw DNJ) was intragastrically administered for 16 weeks, the fecal sterol increased by 22.31%, the bile acid increased by 22.31%, and the SCFAs increased by 36.76%, acetic acid increased by 39.25%, propionic acid increased by 96.52%, butyric acid increased by 60.43%, isobutyric acid increased by 68.05%, valeric acid increased by 74.21%, isovaleric acid increased by 23.78%, and fecal pH decreased by 2.05%. The changes in the indicators were all significant (p<0.05). Serum TC and AI were significantly negatively correlated with acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid (p<0.01). Mulberry leaf water extract can improve the intestinal environment of mice with high-fat diet, promote intestinal microbial fermentation to produce SCFAs, promote the excretion of fecal sterol and bile acid, and thus help reduce blood fat.
