The Anti-hyperglycemic Effects and Mechanism of Egg Oil from Portunus trituberculatus
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Abstract:
The aim of the present study is to investigate the effects and mechanism of egg oil from Portunus trituberculatus (Pt-egg oil) on hyperglycaemia improvement in diabetic mice. The diabetes model rats were established by intraperitoneal injection of streptozotocin. The diabetic rats were given Pt-egg oil at 100 and 400 mg/(kg·body weight) for 60 days, respectively. The fasting blood glucose level, serum insulin concentration, and glucose tolerance were determined. The mRNA expression of the key genes in insulin signaling in skeletal muscle of diabetic rats was measured by the quantitative real time PCR assays. The proteins expression levels in insulin signaling in skeletal muscle of diabetic rats was analyzed by Western blotting. Results showed that Pt-egg oil could significantly reduce fasting blood glucose level by 36.52% (p<0.01), increase serum insulin concentration by 40.21% (p<0.01), and improve glucose tolerance. The phosphatidylinositol 3-hydroxy kinase (PI3K), protein kinase B (Akt), and glucose transporter 4 (Glut4) gene mRNA expression in skeletal muscle increased by 114.52%, 83.17%, and 90.06%, respectively. The protein expression of these three genes increased by 1.54-fold, 1.61-fold, and 1.37-fold, respectively. Glut4 protein expression in membrane was also enhanced by 1.27-fold (p<0.01). These indicated that Pt-egg oil might exhibit the effects on hyperglycaemia improvement via activation of PI3K/Akt insulin signal pathway in diabetic rats.
