Effect of Polypeptide from Moringa oleifera on Antioxidant Capability in D-galactose Induced Aging Mice
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Abstract:
To investigate the effect of polypeptide from Moringaoleracea (MOPP) on the antioxidant capability of D-galactoseinduced aging mice, the aging model was established by intraperitoneal injection of D-galactose (120 mg/kg) in mice. The model mice were divided into normal group, D-galactose aging group, vitamin C group, MOPP low dose (10 mg/kg) and high dose (100mg/kg) group. After 6 weeks of continuous administration, the serum level of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), as well as malondiadehycle (MDA) in serum and main organs including brain, heart and liver were determined by commercial assay kits. The mRNA expressions of Mn-SOD, Gu/Zn-SOD and CAT in liver were determined by qRT-PCR assay. Compared with the aging group, MOPP treatment could significantly improve the serum levels of T-AOC (to 13.73~17.98 U/mL) and activity of SOD(8.04~11.65 U/mg protein), CAT (4.56~6.45 U/mg protein) and GSH-Px (7.28~10.65 U/mg protein) in aging mice. MOPP treatment also increase the activities of SOD, CAT and GSH-Px in main organs (brain, heart and liver) of aging mice (p<0.05). High dose of MOPP also reduced the contents of MDA in the serum, brain, heart and liver revealed a reducing trend by 43.6%, 44.7%, 31.4% and 56.0% (p<0.05), respectively. In addition, MOPP treatment enhanced mRNA transcription of antioxidant factors (SOD, CAT, GSH-Px) in liver of aging mice. The results of this work suggested that MOPP could significantly improve the antioxidant status of D-galactose-induced mice.
