Anti-inflammatory and Immunoactive of Different Solvent Extracts of Lingonberry
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Abstract:
In this work, the effects of different solvent extracts of Vaccinium vitis-idaea L. on the anti-inflammatory and immune functions of inflammatory mice were studied. Continuous oral gavage for 15 days at doses of 100 mg/kg body weight was conducted. The content of glutathione (GSH), malondialdehyde (MDA) and total superoxide dismutase (T-SOD), glutathione reductase (GR) in serum and liver of mice were measured. An ear swelling inflammation model was established to evaluate the inhibition of ear swelling by different solvent extracts. The sepsis model LPS was established to investigate the change in the content of NO and PGE2 by enzyme-linked immunoassay ELISA kit method. The results showed that, for all the four groups treatment, the activity of antioxidant enzymes and the content of GSH increased, whlie the content of MDA decreased. Among them, the GSH content in the n-butanol group was the highest, which increased by 33.47%. The GSH content in the petroleum ether group was the highest in the liver, which increased by 38.61%. For the ethyl acetate group, the T-SOD activity in the blood increase significantly and the MDA decreased by 27.97%. For the content of n-butanol group, the activity of GR in blood and viscera increase significantly by 44.44% and 35.68%, respectively. For all the four groups, the production of NO and PGE2 induced by LPS coukd be inhibited significantly. The effect of inhibition of the ethyl acetate group was slightly worse than the positive control dexamethasone group. This experiment confirmed that Lingonberry extract can not only effectively increase the level of antioxidant enzymes in the body, but also protect cells. It can also reduce PGE2 and NO caused by oxidative stress inflammation. This work provided a theoretical basis for the comprehensive development of lingonberry.
