Nutritional and Lipid Components of Substrate from Isaria cicadae
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Abstract:
In this research, the waste substrate matrix of Isaria cicadae was used as the test material, and its common nutrients were determined using the routine analysis methods. The total lipid content was extracted using an improved chloroform-methanol method, while the polar and neutral lipids in the total fat were separated by silica gel column chromatography. The functional lipid components and fatty acid composition were determined by TLC and gas chromatography. The results showed that the crude protein, total lipid, ash and crude fiber content in the waste substrate matrix of I. cicadae were 15.22, 1.47, 1.33 and 5.54% (on dry mass basis), respectively. The contents of calcium, phosphorus, vitamin A and vitamin E were 0.27%, 0.13% and 266.59 mg/100 g, 62.98 mg/100 g, respectively. TLC analysis revealed that the lipid component contained functional lipids such as sphingomyelin (SM), phosphatidylcholine (PC), phosphatidyl ethanolamine (PE) and triglycerides (TG). The waste substrate matrix of I. cicadae was rich in polyunsaturated fatty acids (accounting for 48.06% of the total fatty acids), with the content of linoleic acid as high as 47.94%. I. cicadae contained 17 types of amino acids with the total amount being 88.27 mg/g (out of which essential amino acids accounting for 53.11%). In summary, the waste substrate matrix of I. cicadae is rich in various nutrients and can be used for food or animal feed applications.
