Activities of Active Oxygen Metabolism Enzymes and Isoenzymes in Apricot Fruit Treated with 1-MCP and Ethephon
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Abstract:
Using Luntai Baixing as test material, 1 μL/L of 1-methylcyclopropene (1-MCP) and 30 mg/kg of ethephon were used to treat apricot fruit, the aim is to study the changes in the activities of superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD) and lipoxygenase (LOX) in apricot pulp stored at 25 ℃, and their isoenzyme expression changes. The results showed that the 1-MCP treatment group significantly delayed the aging and softening of apricot fruit, and the ethephon treatment accelerated the senescence and softening of apricot fruit. The enzyme activities of SOD, PPO and POD in the apricot fruit treated by 1-MCP were higher than those in the control and ethephon treatment groups. The activity of SOD was 25.21% higher than that of the control group (p<0.05), the activity of PPO was 12.93% higher than that of the control group (p<0.05), the activity of POD was 9.96% higher than that of the control group (p<0.05), and the activity of LOX was significantly lower than that of the control group, and the ethephon treatment group was 46% lower than the control group (p≥0.05). The expression of SOD, PPO and POD isoenzymes in the 1-MCP treatment group and the control group were enhanced, and the expression of LOX isoenzyme was reduced. As the storage time increased, the isozyme bands changed from clear to diffuse band, and isozyme expression decreased with time. 1-MCP reduced the activity of active oxygen metabolism and the production of reactive oxygen species, slowed down the process of cell aging, delayed softening, and improved the storage quality of apricot fruit.
