High-throughput Sequencing Analysis of Probiotics Intervention in Intestinal Flora of Cold-fed Mice
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Abstract:
High-throughput sequencing was used to analyze the effects of probiotics on the intestinal flora of cold-fed mice. The experimental mice were randomly divided into a model group, a control group, and a low, medium, and high dose group, and an equal amount of ordinary ice cream, 107 cfu/mL probiotic liquid, and 107, 108, 109 cfu/mL probiotic ice cream, respectively. The rats were continuously fed for 15 days, and the stool rate and diarrhea index of mice were recorded and calculated. After 15 days, the feces of each group were collected for high-throughput sequencing analysis. It was found that cold food could cause diarrhea in some mice. In the dose range, probiotics could alleviate the symptoms of diarrhea in cold-sensitive mice. The high-dose group decreased the stool rate by 3.11% and the diarrhea index (10-1) by 0.33. The inhibition effect was significant, compared to the other groups. The fecal flora of mice with probiotics was highly diverse (the high-dose group had an ACE value of 69987.29 and the Chao1 value was 27098.77), and the proportion of genus Lactobacillus, Ekmenia, and Blautia increased and their structure changed. Therefore, probiotics could improve the acceptance of cold food in cold-sensitive diarrhea-sensitive mice. The probiotics under the frozen carrier could be colonized in the intestine of mice and regulate the intestinal tract of mice.
