Blueberry Anthocyanin Extracts Regulate Glucose Consumption in HepG2 Cells
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Abstract:
The regulation of glucose consumption in insulin-resistant hepatoma cell line HepG2 with blueberry anthocyanin extracts was investigated. The optimum concentration of blueberry anthocyanin extracts was screened by MTT assay. Different concentrations of insulin and glucose were performed to induce insulin resistance in HepG2 cells. The number of cells was corrected by MTT, and contents of glucose in medium were detected by glucose oxidase method. Changes in glucose in the medium reflected the intervention of the blueberry anthocyanin extract on cellular insulin resistance. MTT results showed that and there was no significant effect on the activity of HepG2 cells at concentrations below 200 μg/mL of the extracts of NL BAEs and that below 100 μg/mL of the extracts of BW BAEs and GB BAEs. With the inducing agent insulin concentration of 1×10-8 mol/L and incubation for 24 h as the induction condition, the model was effective, while the cell viability had no significant change and the stability was good. It could promote glucose consumption in normal HepG2 cells and insulin-resistant HepG2 cells at the concentration of 7.8125~31.25 μg/mL of three varieties of blueberry anthocyanin extracts and showed a dose-effect relationship. It had a maximum at 31.25 μg/mL. Compared to the model group, sugar consumptions of extracts of NL BAEs, BW BAEs and GB BAEs had increased by 60%, 65% and 88%, respectively. Three varieties of blueberry anthocyanin extract could prevent and improve the utilization of glucose by insulin-resistant HepG2 cells.
